Does Buffalo River hold wild trout?

Yes. The state classifies Buffalo River as Class II (some natural reproduction) across 13.09 miles of stream. That classification means natural reproduction, not stocking. Source: Wisconsin DNR Classified Trout Streams.

Buffalo River

Wisconsin. Classified Class II (some natural reproduction) by the state across 13.09 miles.

State Trout Classification

Classification	Class II (some natural reproduction)
Classified length	13.09 miles

Source: Wisconsin DNR Classified Trout Streams.

Fly fishing rivers →Wisconsin trout water →Wisconsin lakes →

Conditions: USGS NWIS (public domain). Classification: Wisconsin DNR Classified Trout Streams.